Fig. 4. Seahorse peptide attenuates uPA and plasminogen-dependent invasive migration of HT1080 cells. (A) The 0.1 M peptide treatment decreased uPA expression. (B, C) HT1080 cells were transfected with uPA or plasminogen siRNA. After transfection with siRNA, the cells were incubated for 48 h in the absence or presence of 0.1 M peptide and then the cells were analyzed by western blot or harvested for invasive migration assays. All data are expressed as the average of three different experiments. *P < 0.05 compared to controls RNAi.
