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KO2 experiments in 50 mM NaOH. (A) O2•− total spin concentration based on double integration of obtained EPR spectra. For details, see Methods. (B) The turnover number (moles of superoxide per mole of PEG-HCCs per second) was calculated by subtracting the amount of remaining O2•− from the amount of O2•− in the control, dividing by the amount of PEG-HCCs and then by 15 s for the reaction time (the average time required for manually freeze-trapping each EPR sample).
