Fig. 5.

Derived rate constants from simulated plots using the same inputs as in Fig. 4 vs. protein concentration for time courses of 20, 40, 80, and 100 min (0.1–10 µM protein for k₁ and 0.03–10 µM for k₂). Concentration dependence of rate constants (A) k₁, (B) k₂, (C) k₁k₂, and (D) initial aggregation rate. Time courses over 20 and 40 min were fitted to Eq. 1 without the linear drift term, which was included for 80 and 100 min. The term a was included to mimic the fitting of data in experiments. The values of k₁ and k₂ plotted are the observed values from fitting aggregation curves given by Scheme 2 with Eq. 1 as shown in Fig. 4 and not the microscopic values in Scheme 2.