Fig. 3.

OsMADS15 protein localization in the rice shoot apex during the transition from vegetative to reproductive stage. Confocal images of OsMADS15–mOrange gene targeting plants at the indicated stages are shown. Stages and abbreviations are as described in the Fig. 1 legend. (A, B, E, and F) V, vegetative-stage plants at 27 DAG. (C and G) R1. (D and H) R2. (I, L, M, and P) R3. (J and N) R4. (K and O) Fl. (L) Enlarged view of the R3 stage meristem (red square in P) to illustrate the accumulation of mOrange in the nuclei. (Scale bars, 50 µm.) (Q) Schematic diagrams of OsMADS15–mOrange gene targeting with representative features. Dark gray boxes, exons; light gray boxes, UTRs. The direction of transcription is indicated by arrows. Orange pentagon, mOrange coding sequence; red arrow, expression cassette of hygromycin phosphotransferase (hpt) for selection of targeting allele.