Table 4.1.
Precision, accuracy, matrix effect, and recovery data of 21 bile acids in rat serum samples
| BAs | concentration | intra-day (n=6) | inter-day (n=5-6) | matrix effect | recovery | |||
|---|---|---|---|---|---|---|---|---|
| ng/mL | CV (%) |
accuracy (%) |
CV (%) |
accuracy (%) |
(n=6, %) | (n=6, %) | ||
| C | 5 | 16.4 | 95.3 | 3.9 | 82.0 | 105±14.9 | 109±8.4 | |
| 50 | 4.3 | 95.9 | 4.7 | 94.3 | 97.2±3.2 | 109±1.4 | ||
| 500 | 5.2 | 103 | 4.2 | 104 | 91.7±1.6 | 110±4.1 | ||
| TC | 5 | 22.8 | 102 | 26.4 | 63.2 | 110±30.2 | 141±24.2 | |
| 50 | 4.5 | 104 | 24.2 | 91.4 | 126±12.7 | 113±9.8 | ||
| 500 | 3.3 | 100 | 17.2 | 87.1 | 132±9.0 | 110±13.5 | ||
| GC | 5 | 16.3 | 122 | 20.8 | 80.2 | 220±35.4 | 65.3±10.4 | |
| 50 | 7.4 | 94.2 | 22.8 | 125 | 122±22.1 | 94.0±24.7 | ||
| 500 | 12.0 | 89.9 | 27.5 | 125 | 81.1±12.1 | 148±14.1 | ||
| CDC | 5 | 11.8 | 88.8 | 11.7 | 100 | 88.4±11.3 | 129±15.5 | |
| 50 | 14.5 | 108 | 3.1 | 94.5 | 79.6±4.2 | 101±8.9 | ||
| 500 | 9.4 | 102 | 9.5 | 95.7 | 110±9.5 | 91.1±17.7 | ||
| TCDC | 5 | 6.4 | 83.7 | 18.3 | 77.6 | 128±21.6 | 141±24.2 | |
| 50 | 5.2 | 98.4 | 11.3 | 88.0 | 143±18.9 | 121±16.6 | ||
| 500 | 3.6 | 100 | 13.4 | 86.9 | 171±25.6 | 116±20.5 | ||
| GCDC | 5 | 8.4 | 90.7 | 14.7 | 82.4 | 88.6±7.1 | 116±10.0 | |
| 50 | 6.1 | 104 | 12.4 | 98.5 | 106±4.9 | 106±6.4 | ||
| 500 | 4.3 | 101 | 7.3 | 96.7 | 101±8.1 | 114±7.6 | ||
| UDC | 5 | 2.7 | 88.5 | 4.6 | 83.1 | 80.0±6.9 | 130±12.0 | |
| 50 | 5.2 | 110 | 6.4 | 94.9 | 89.3±4.6 | 111±7.6 | ||
| 500 | 3.9 | 97.3 | 6.8 | 87.9 | 102±7.4 | 102±6.9 | ||
| TUDC | 5 | 10.2 | 103 | 26.7 | 69.2 | 117±19.9 | 146±32.8 | |
| 50 | 4.1 | 105 | 21.2 | 90.4 | 133±15.7 | 113±9.8 | ||
| 500 | 3.5 | 99.9 | 16.1 | 87.8 | 142±10.8 | 111±14.3 | ||
| GUDC | 5 | 7.0 | 95.3 | 6.2 | 74.8 | 106±9.6 | 117±15.4 | |
| 50 | 4.1 | 106 | 12.5 | 93.3 | 99.4±8.3 | 110±14.1 | ||
| 500 | 4.3 | 108 | 9.0 | 108 | 94.8±19.6 | 140±28.2 | ||
| DC | 5 | 18.7 | 106 | 8.9 | 103 | 85.5±9.5 | 116±31.0 | |
| 50 | 8.9 | 97.2 | 7.5 | 98.6 | 85.1±4.8 | 97.3±11.1 | ||
| 500 | 9.0 | 98.8 | 12.1 | 98.9 | 66.6±6.0 | 121±11.8 | ||
| TDC | 5 | 4.5 | 89.3 | 16.7 | 76.0 | 145±19.8 | 138±24.4 | |
| 50 | 5.8 | 110 | 13.5 | 88.2 | 154±21.2 | 121±18.4 | ||
| 500 | 8.4 | 103 | 13.5 | 95.7 | 189±22.7 | 114±14.4 | ||
| GDC | 5 | 3.6 | 93.5 | 14.0 | 71.6 | 97.2±8.3 | 116±11.0 | |
| 50 | 6.2 | 99.5 | 9.0 | 95.8 | 102±5.0 | 107±9.2 | ||
| 500 | 5.6 | 102 | 8.9 | 99.4 | 103±5.0 | 114±8.1 | ||
| LC | 5 | 7.0 | 175 | 17.9 | 186 | 68.0±4.1 | 94.1±10.7 | |
| 50 | 11.9 | 116 | 15.5 | 109 | 62.8±8.2 | 85.5±19.4 | ||
| 500 | 17.2 | 106 | 29.5 | 98.3 | 89.7±17.4 | 88.9±19.7 | ||
| TLC | 5 | 7.1 | 84.1 | 25.5 | 66.6 | 241±53.7 | 131±36.2 | |
| 50 | 6.9 | 104 | 10.1 | 83.9 | 261±23.4 | 110±10.1 | ||
| 500 | 4.4 | 97.6 | 11.3 | 83.9 | 330±30.0 | 100±11.8 | ||
| GLC | 5 | 17.8 | 103 | 17.8 | 107 | 107±6.8 | 97.7±7.6 | |
| 50 | 15.5 | 125 | 14.8 | 139 | 147±17.4 | 96.7±24.2 | ||
| 500 | 7.9 | 111 | 17.5 | 137 | 117±9.2 | 85.6±18.7 | ||
| DHC | 5 | n.d.* | n.d.* | n.d.* | n.d.* | n.a.† | n.a.† | |
| 50 | 7.0 | 90.5 | 22.2 | 112 | 98.6±13.8 | 82.8±16.4 | ||
| 500 | 11.6 | 82.8 | 26.7 | 93.9 | 71.8±5.3 | 116±18.6 | ||
| TDHC | 5 | 15.2 | 106 | 17.2 | 53.4 | 52.9±5.3 | 133±20.2 | |
| 50 | 10.1 | 100 | 17.7 | 53.1 | 58.0±2.3 | 107±9.5 | ||
| 500 | 8.6 | 104 | 15.1 | 59.2 | 65.1±7.5 | 127±22.1 | ||
| GDHC | 5 | 10.2 | 87.8 | 7.7 | 56.8 | 80.0±9.8 | 116±19.4 | |
| 50 | 8.7 | 94.1 | 16.3 | 69.2 | 68.1±5.4 | 105±10.6 | ||
| 500 | 4.7 | 82.8 | 9.5 | 69.5 | 59.5±3.7 | 118±4.6 | ||
| αMC | 5 | 9.0 | 84.6 | 7.0 | 69.4 | 87.2±5.9 | 104±8.0 | |
| 50 | 2.6 | 96.6 | 2.6 | 92.6 | 77.0±13.9 | 113±29.9 | ||
| 500 | 6.3 | 97.9 | 4.5 | 84.4 | 79.9±14.1 | 122±23.0 | ||
| βMC | 5 | 6.4 | 121 | 7.3 | 78.7 | 82.2±8.7 | 132±14.8 | |
| 50 | 3.8 | 104 | 8.8 | 89.0 | 79.2±14.7 | 122±30.7 | ||
| 500 | 3.7 | 99.3 | 6.7 | 88.4 | 93.9±14.5 | 112±20.6 | ||
| HDC | 5 | 9.0 | 115 | 5.8 | 105 | 81.9±9.6 | 123±10.8 | |
| 50 | 5.9 | 112 | 7.7 | 99.5 | 91.4±7.1 | 111±12.0 | ||
| 500 | 2.6 | 97.4 | 7.7 | 101 | 103±9.9 | 104±8.9 | ||
*: Not detected
†: Not applicable