Table 4.2.

Precision, accuracy, matrix effect, and recovery data of 21 bile acids in rat liver samples

BAs	concentration	intra-day (n=6)			inter-day (n=6)		matrix effect	recovery
	ng/g liver	CV (%)	accuracy (%)		CV (%)	accuracy (%)	(n=6, %)	(n=6, %)
C	50	2.6	99.1		3.3	98.8	103±5.0	98.6±6.5
	500	2.5	91.0		5.6	91.0	93.8±5.7	106±6.4
	5000	1.1	103		1.8	102	103±12.5	97.7±11.4
TC	50	8.0	104		5.6	111	105±9.0	113±9.3
	500	2.2	112		6.3	121	123±5.6	98.6±6.2
	5000	2.0	114		3.5	117	130±13.8	99.2±12.0
GC	50	3.1	92.1		7.0	111	87.3±9.3	91.7±9.2
	500	4.4	101		8.8	116	81.0±5.0	104±8.4
	5000	4.0	78.5		5.4	85.7	98.7±13.6	96.0±11.2
CDC	50	14.0	100		14.2	107	65.0±11.4	123±14.4
	500	4.4	78.6		9.5	87.7	111±10.6	95.1±13.1
	5000	3.3	94.9		4.6	90.9	94.3±19.3	102±16.9
TCDC	50	4.0	106		3.4	106	172±6.4	97.3±4.1
	500	3.3	119		4.8	120	150±3.3	104±3.7
	5000	1.0	107		2.7	110	144±14.7	100±13.1
GCDC	50	3.7	91.6		3.2	94.8	108±5.9	96.1±9.4
	500	2.9	102		5.9	106	99.7±4.2	105±5.6
	5000	2.9	85.1		1.9	109	104±16.3	98.9±13.8
UDC	50	9.0	102		7.6	103	68.5±8.1	134±11.6
	500	2.9	111		7.1	109	99.2±6.4	101±8.3
	5000	2.2	105		3.6	105	106±9.3	93.6±9.7
TUDC	50	6.7	95.8		4.4	106	114±6.1	97.9±8.2
	500	4.1	112		6.4	118	125±5.9	106±9.7
	5000	2.6	111		2.4	89.7	131±15.2	99.5±12.5
GUDC	50	2.2	91.3		7.7	98.8	81.6±8.7	102±7.7
	500	3.5	109		7.2	118	85.3±6.9	107±8.1
	5000	4.5	101		4.0	105	104±14.3	101±11.0
DC	50	3.5	84.7		8.4	97.2	97.1±16.4	86.0±19.8
	500	6.0	107		14.1	108	95.4±8.3	99.7±5.2
	5000	6.4	83.0		13.3	107	105±6.0	83.1±7.6
TDC	50	3.7	113		6.5	106	170±6.5	103±4.2
	500	2.8	113		5.9	120	163±3.1	101±4.4
	5000	1.4	110		2.5	113	153±15.7	102±13.3
GDC	50	3.9	101		2.8	97.4	112±19.0	101±20.5
	500	3.0	110		4.8	87.5	106±5.4	107±6.3
	5000	1.9	101		3.2	103	114±15.1	99.5±12.8
LC	50	15.3	90.3		11.2	84.8	67.4±10.0	108±17.8
	500	11.9	91.8		16.7	96.1	102±15.1	70.8±14.5
	5000	6.9	100		6.9	97.9	98.6±11.6	69.0±16.7
TLC	50	5.6	116		9.3	103	368±7.6	84.2±12.3
	500	5.4	103		5.7	113	260±4.9	97.1±6.0
	5000	3.4	89.9		5.8	95.9	242±16.5	91.8±15.5
GLC	50	12.5	90.1		8.6	96.0	166±14.0	74.3±11.7
	500	8.1	109		14.2	108	93.5±17.4	104±13.0
	5000	8.4	99.6		9.5	106	114±12.3	81.6±9.6
DHC	50	19.9	91.0		25.9	180	28.5±34.8	191±28.3
	500	3.0	115		4.0	117	84.4±19.5	88.0±21.8
	5000	7.1	130		7.0	133	89.1±3.7	76.7±6.9
TDHC	50	18.0	119		9.9	114	43.7±15.2	121±25.5
	500	5.9	140		7.1	140	75.1±11.3	103±14.9
	5000	5.4	83.2		9.0	88.3	91.7±6.7	88.4±8.6
GDHC	50	14.3	92.2		6.2	101	41.5±13.6	94.9±24.9
	500	10.7	101		7.5	109	48.4±10.0	94.9±18.2
	5000	11.0	110		3.0	87.8	56.8±14.6	86.5±15.1
αMC	50	4.0	94.2		3.5	94.6	77.7±6.3	105±9.4
	500	2.4	110		6.4	111	85.1±5.5	105±6.9
	5000	3.2	94.0		2.4	93.5	89.9±12.8	97.8±11.0
βMC	50	3.4	99.5		3.5	96.7	83.6±6.1	114±8.2
	500	2.0	111		6.4	109	89.9±7.4	104±8.2
	5000	3.1	104		2.8	102	98.6±10.7	95.8±9.7
HDC	50	8.0	108		7.6	101	58.3±13.2	150±13.1
	500	1.5	112		5.0	108	99.1±8.1	98.8±8.6
	5000	1.9	93.0		3.2	95.7	109±9.7	93.5±9.1

*: Not detected