Table 3.
Validation of selected array-based gene expression by real-time RT-PCR analysis.
| Probe ID | Description | Microarray | qRT–PCR | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 0.5 h/0 h | 2 h/0 h | 24 h/0 h | 5 d/0 h | 0.5 h/0 h | 2 h/0 h | 24 h/0 h | 5 d/0 h | ||
| GO_dr004P0029M08_F_ab1 | CBF1 | 3.58 | 6.66 | 5.53 | 4.35 | 5.41 | 6.07 | 6.83 | 3.73 |
| cn9604 | AP2-EREBP | 1.21 | 2.56 | 1.88 | 0.64 | 3.30 | 4.12 | 3.63 | 2.27 |
| GO_dr001P0015C16_F_ab1 | AP2-EREBP | 0.52 | 1.32 | 2.00 | 1.65 | 2.32 | 3.14 | 2.68 | 3.40 |
| GO_drpoolB-CL3762Contig1 (PhZFP1) | Zinc finger protein (C2H2-type) | 2.53 | 3.68 | 2.73 | 1.02 | 3.08 | 3.41 | 3.45 | 1.35 |
| cn1485 | Zinc finger protein (C2H2-type) | 0.98 | 1.17 | 1.34 | 1.22 | 3.40 | 4.31 | 4.74 | 3.83 |
| GO_dr004P0009O19_F_ab1 | Zinc finger protein (RING-finger) | −0.31 | −0.73 | −1.87 | −2.37 | −0.55 | −0.36 | −0.62 | −4.39 |
| GO_drs31P0004P18_R_ab1 | Zinc finger protein(C3H-type) | 0.55 | 2.19 | 2.63 | 1.54 | 2.95 | 3.74 | 4.77 | 3.23 |
| cn9788 | RING-H2 subgroup RHE protein | 1.29 | 2.07 | 1.62 | 0.62 | 2.98 | 4.09 | 3.64 | 0.88 |
| cn6897 | CCR4 associated factor 1-related protein | 1.39 | 2.29 | 1.34 | 0.47 | 3.90 | 4.63 | 3.33 | −0.11 |
All data are shown log2 Ratio, and positive and negative values of Log2 Ratio are either up- or down-regulated genes in the four pair-comparisons.