Table 1.
The assay performance of selected microRNAs.
| Gene name | Accession | Sequence | Length (nt) | PCR efficiency (%) | Intra-assay Precision | |
|---|---|---|---|---|---|---|
| Mean CV (%) | Min-Max (%) | |||||
| let-7a | MIMAT0000062 | UGAGGUAGUAGGUUGUAUAGUU | 22 | 98 | 0.38 | 0.01–1.41 |
| miR-106a | MIMAT0000103 | AAAAGUGCUUACAGUGCAGGUAG | 23 | 96 | 0.41 | 0.08–1.95 |
| miR-155 | MIMAT0000646 | UUAAUGCUAAUCGUGAUAGGGGU | 23 | 99 | 0.38 | 0.04–1.09 |
| miR-16 | MIMAT0000069 | UAGCAGCACGUAAAUAUUGGCG | 22 | 97 | 0.51 | 0.04–2.05 |
| miR-21 | MIMAT0000076 | UAGCUUAUCAGACUGAUGUUGA | 22 | 97 | 0.36 | 0.04–1.69 |
| miR-219 | MIMAT0000276 | UGAUUGUCCAAACGCAAUUCU | 21 | 95 | 1.61 | 0.09–4.49 |
| miR-221 | MIMAT0004568 | ACCUGGCAUACAAUGUAGAUUU | 22 | 96 | 1.71 | 0.11–6.23 |
| U6-snRNA | NR_002752.2 | Omitted due to length | 107 | Not detected | 0.44 | 0.01–0.99 |
CV – coefficient of variation.