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. 2015 Mar 2;8:1. doi: 10.3389/fneng.2015.00001

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Copyright © 2015 Avants, Murphy, Dapello and Robinson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Neuron expressing ChR2-GFP was stimulated by blue light (470 nm) illumination with patterns I (magenta) and II (cyan). (B) The depolarization in response to 20 ms (blue bar) stimulation was recorded by whole cell patch-clamp electrophysiology. The depolarization in response to stimulating two ROIs simultaneously (solid black I and II) is greater than the summed depolarization of I and II stimulated individually (dashed black I + II). Data is averaged from three trials; scale bars: (A) 25 um, (B) 4 mV and 20 ms.