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. 2015 Jan 8;74(3):569–578. doi: 10.1136/annrheumdis-2014-205743

Figure 4.

Figure 4

Peroxisome proliferator-activated receptor gamma (PPARγ) expression vector rescues the aberrant expression of catabolic, anabolic and inflammatory markers, and the dysregulated expression of mammalian target for rapamycin (mTOR)/autophagy markers in PPARγ knockout osteoarthritis (KO OA) chondrocytes: PPARγ KO chondrocytes (isolated at 5 weeks post-OA surgery) were cultured and transfected with PPARγ expression vector or empty vector. Rescue of PPARγ in PPARγ KO chondrocytes by PPARγ expression vector resulted in: (A and B) decrease in the protein and messenger RNA expression of mTOR (n=4, *p<0.05); (C) increase in LC3B II expression and decrease in MMP-13 expression (n=4), (D) increase in the mRNA expression of LC3B and decrease in the mRNA expression of MMP-13; (E) increase in mRNA expression of ULK1, ATG5 and BNIP3; (F) increase in the expression of aggrecan and collagen type II and decrease in the mRNA expression of ADAMTS-5; (G) decrease in mRNA expression of COX-2 and iNOS; (n=4, *p<0.05).