Skip to main content
. 2013 Nov 18;15(2):157–178. doi: 10.1111/tra.12134

Figure 1.

Figure 1

Requirement for human Rab GTPases in HSV1 infection. A) siRNAs from a library against 60 human Rabs were transfected into HeLa cells, alongside a control negative siRNA, pooling siRNAs to different isoforms of the same Rab. Two days later, the transfected cells were infected with HSV1 at a multiplicity of 2, and extracellular released virus harvested at 16 h and titrated on Vero cells. Error bars indicate standard error from three independent experiments. B) The graph from (A) is presented as the relative log drop in virus yield, in order from the largest to the smallest. Error bars indicate standard error from three independent experiments. Statistical analysis was carried out using the Student's t-test. ***p < 0.001; **p < 0.01. C) HeLa cells were transfected with negative or Rab6A siRNAs or left untransfected, and infected as above. Five hour after infection BFA (1 µg/mL) was added to one untransfected sample, and released virus harvested from all samples at 18 h. Error bars indicate standard error from three independent experiments. D) HeLa cells were transfected with the negative siRNA or pooled siRNAs to Rab1A and1B, Rab6A, 6B and 6C or Rab24 and analysed 2 days later by western blotting for these Rabs. Alpha tubulin (tub) was used as the loading control. Molecular weight markers are shown in kDa.