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. 2013 Nov 18;15(2):157–178. doi: 10.1111/tra.12134

Figure 2.

Figure 2

Rab6 depletion inhibits virus production at late stages of infection. A) HeLa cells transfected with neg, Rab1 or Rab6 siRNAs were infected with HSV1 2 days later and harvested 16 h after infection for western blotting with antibodies as indicated. Mature glycosylated forms of gD and gE are marked by asterisk. B) The status of glycoprotein glycosylation in control (neg), Rab6 depleted or monensin treated cells was determined by Endo H treatment of infected cell lysates harvested at 16 h, and analysed by western blotting for gD. Three isoforms of gD are denoted by 1, 2 & 3. C) The level of DNA replication in control (neg), Rab6 depleted or Ara C treated cells was analysed by semi-quantitative PCR using a primer pair specific for the UL47 gene. PCR cycle numbers are denoted. D) Hela cells transfected with neg or Rab6 siRNAs were infected 2 days later with HSV1, and cell-associated and released virus titrated on Vero cells. Molecular weight markers are shown in kDa.