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. 2013 Nov 18;15(2):157–178. doi: 10.1111/tra.12134

Figure 7.

Figure 7

The Rab6 fission effector non-muscle myosin II is not required for HSV1 morphogenesis. A) HeLa cells infected with HSV1 at a multiplicity of 2 were treated with blebbistatin at 5 h or left untreated and harvested for western blotting at 16 h. B) HeLa or HFFF cells infected with HSV1 at a multiplicity of 2 were treated with blebbistatin at 5 h or left untreated and harvested for extracellular virus yield at 18 h. C and D) HeLa cells were transfected with neg, myosin IIA (IIA) or myosin IIB (IIB) siRNAs, infected 2 day later with HSV1 at a multiplicity of 2, and harvested for western blotting (C) or extracellular virus (D). E) HeLa cells on coverslips were transfected with neg or myosin IIB (NMIIB) siRNAs and infected 2 days later with Wt HSV1. Eight and sixteen hours later the cells were processed for gD detection at the cell surface, and stained with secondary antibody (red). Nuclei were stained with DAPI (blue). Scale bar = 10 µm. Error bars indicate standard error from three experiments. Molecular weight markers are shown in kDa.