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. 2013 Nov 18;15(2):157–178. doi: 10.1111/tra.12134

Figure 8.

Figure 8

The Rab6 effector ERC1 facilitates HSV1 glycoprotein trafficking and morphogenesis. A–C) HeLa cells were transfected with neg siRNA or siRNA to bicaudal D1 (BicD1), bicaudal D2 (BicD2), conventional kinesin heavy chain (KIF5B), dynactin 1 (Dyn), ERC1 or rabkinesin 6 (KIF20A) and 2 days later were either harvested for western blotting with antibodies specific to each effector (A) or infected with HSV1 at a multiplicity of 2, and harvested at 16 h for western blotting for the virus proteins ICP0 and gD (B), or extracellular virus (C). Error bars indicate standard error from three experiments. D) HeLa cells on coverslips were transfected with neg or ERC1 siRNAs and infected 2 days later with HSV1. Eight or sixteen hours later the cells were processed for detection of cell surface gD, and stained with secondary antibody (red). Nuclei were stained with DAPI (blue), and images were acquired with the same acquisition settings. Scale bar = 10 µm. Molecular weight markers are shown in kDa.