Figure 2.

Flow cytometry evaluation using annexin V FITC/PI, showing the effects of 20 μM EGCG treatment for 24 hours and 48 hours on SSC-4 cells.

Notes: Viable cells are located in the lower left quadrant, and early apoptotic cells are positioned in the lower right quadrant of the two flow cytometric displays, while late apoptotic cells are located in the upper right quadrant, and necrotic cells are displayed in the upper left quadrant (24 hours). The image at the right presents the % apoptotic, necrotic, and viable cells, from total numbers of cells.

Abbreviations: EGCG, epigallocatechin-3-gallate; FITC, fluorescein isothiocyanate; PI, propidium iodide.