Figure 6.

Single-molecule super-resolution imaging setup. 405- and 561-nm laser light is selected with excitation filters, circularly polarized with quarter waveplates, and controlled with a pair of shutters. The beams are coupled with a dichroic mirror. The laser beams are focused onto the back aperture of a high-NA microscope objective and the sample is excited by epi-illumination. Emitted fluorescence is filtered using an emission filter and a second dichroic. This emission is magnified with a beam expander if required and then detected on an EMCCD camera.