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. 2014 Aug 13;19(8):12116–12149. doi: 10.3390/molecules190812116

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© 2014 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(a) Time-lapse images of diffusing TcpP-PAmCherry molecules in live V. cholerae cells. The arrowhead highlights one molecule, which turns on in the fourth panel and moves inside the cell. Each panel is 50 ms. Scale bar: 1 µm; (b) Implementing a step size threshold (dashed line). Top: The black molecule cannot be connected to either molecular position in the next frame because they are both beyond the maximum step size. Bottom: If more than one localization is within the threshold, the nearest is chosen.