(A) Staining of Dorsal and nuclear membrane (WGA) in the fat body of wild type (w1118) and tubGal4-dvps35RNAi third instar larvae. Scale bar, 20 μm. (B) Staining of DIF and DAPI in the adult fat body of wild type (CS) and dvps35 mutant. CS, Canton-S. (C) Staining of F-actin, Dorsal and phospho-Histone H3 in hemocytes of tubGal4 and tubGal4-dvps35RNAi third instar larvae. P, plasmatocytes; L, lamellocytes; red dotted circles, nuclei. Scale bar, 10 μm. (D) Ratio of hemocytes with Dorsal in the nuclei in tubGal4 and tubGal4-dvps35RNAi third instar larvae.