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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jan 29;458(1):214–219. doi: 10.1016/j.bbrc.2015.01.093

Fig. 3. Effect of GSNO treatment on GSK-3β activation in Aβ25–35 treated neurons.

Fig. 3

A. Effect of GSNO (100µM) and Aβ25–35 (40µM) treatment on the activities of GSK-3β was analyzed in a time course manner in primary cultured cortical neurons. B and C. To examine the effect of GSNO on Aβ25–35-induced GSK-3β activities, the cultured neurons were pretreated with GSNO for 4hrs and treated with Aβ25–35 for 12 hrs. GSK-3β enzyme activities were analyzed by in vitro kinase assay (B) or Western blot by using antibodies specific to phospho-GSK-3α/β (Tyr279/216; p-GSK-3α-Y279, p-GSK-3β-Y216) and phospho-GSK-3β (Ser9; p-GSK-3β-S9), and total GSK-3β (C). The vertical columns are means of individual data and T-bars are standard error mean. *p<0.01; and **p<0.005 as compared to the control group. +p<0.01 as compared to Aβ treated groups.