Figure 2.

Scatterplot and histogram showing percentage of cells with non-fused break-apart signals detected by fluorescence in-situ hybridization (FISH) in each of the two cases of colon and gastric adenocarcinoma employed in this study. There was a single case out of the 42 cases (2.3%) that was positive for anaplastic lymphoma kinase (ALK) rearrangement by FISH using the standard criteria of ⩾15% of cells being positive for the break-apart signal. Using a less-restrictive cut-off of 10% positive cells, 7 cases (16%) were considered either positive or possibly positive.