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. 2015 Mar 2;10(3):e0117224. doi: 10.1371/journal.pone.0117224

Fig 1. Immunophenotype of human anterior cruciate ligament (ACL)-derived mesenchymal stem cells (MSCs) from total knee arthroplasty (TKA) and ACL reconstruction.

Fig 1

Cells from TKA and ACL reconstruction samples at (A) passage 0, (B) passage 1 and (C) passage 2 were stained with antibodies against CD34, CD44, CD90 and CD105 and analyzed by flow cytometry. CD34 negative populations were gated in live populations (P1); CD44 positive populations were regated in CD34 negative populations; CD90 and CD105 positive populations were regated in CD44 positive populations. Representative histograms are shown in blue lines while red lines represent isotype controls.