Figure 3. Soluble and insoluble Aβ is not affected by Aβ immunization and LXR agonist treatment.
Soluble Aβ was extracted from cortexes and hippocampi by TBS followed by extraction of insoluble using formic acid, and Aβ was evaluated by ELISA. On panel A is shown insoluble Aβ40 and Aβ42 and on panel B, soluble Aβ40 and Aβ42. C, The level of Aβ oligomers was measured in soluble fraction using dimer-specific ELISA as described in the methods. For all panels, difference between the means were analyzed by one-way ANOVA and showed no significant change and two-way ANOVA demonstrated no interaction and no significant main effects of LXR or anti- Aβ treatments. N=6–14 male and female mice per group.
