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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: J Alzheimers Dis. 2014;41(2):535–549. doi: 10.3233/JAD-132789

Figure 5. Immunization and LXR agonist decrease Aβ level in ISF and increases ApoE lipidation.

Figure 5

APP23 mice were treated with HJ3.4 antibody plus/minus LXR agonist T0 for 15 days. ISF was recovered by microdialysis and Aβ level determined by ELISA as described in the text. A, Aβ40 and B, Aβ42. Means were compared by one-way ANOVA and Dunnett post-test. * < 0.05 and **, p < 0.01 versus IgG. Two-way ANOVA analysis of Aβ42 levels demonstrates that there is no interaction between immunization and T0 and significant single effects of anti- Aβ F(1,7)=6.42, p=0.034 and T0 F(1,7)=.23.56, p=0.0018. C, The level of Aβ dimers was measured by dimer specific ELISA as described in the methods. For A, B and C, N=3 mice per group. N.S. is non-significant. D, Native gel electrophoresis demonstrated an increase of ApoE lipidation in ISF after T0 treatment. For comparison, on the left is shown 1 µJ wild type mouse sera exposed for a shorter time. For all panels N=3 mice per group. N.S. is non-significant.