Figure 4. Effect of Tim-1 expression or defects in B cells on EAE and T cell responses.

A) WT total CD4⁺ T cells (10 × 10⁶/mouse) were co-transferred together with either WT or Tim-1^−/− CD19⁺ B cells (20 × 10⁶) into Rag1^−/− mice; the recipients were then immunized with MOG35-55/CFA to induce EAE. Mice were scored daily for clinical signs of EAE (left panel; n =10 per group). * P < 0.05. On day 20, CNS-infiltrating mononuclear cells were isolated and examined for the frequencies of IFN-γ⁺, IL-17⁺, and Foxp3⁺ cells in CD4⁺ T cell gates by flow cytometry after intracellular staining (middle panel; n = 5). CNS-infiltrating CD4⁺ T cells were also isolated and measured for their IL10 mRNA expression by realtime PCR (right panel; ** P < 0.01; n = 4 per group). B) WT total CD4⁺ T cells (10 × 10⁶) were co-transferred together with WT (20 × 10⁶), Tim-1-^−/− (20 × 10⁶), or Tim-1^−/− (20 × 10⁶) plus WT Tim-1⁺ (2 × 10⁶) B cells into Rag1^−/− mice; the recipients were then immunized with MOG35-55/CFA to induce EAE. Mice (n = 8-10 per group) were scored daily for clinical signs of EAE. * P < 0.05.