Fig. 4.

Inhibition of TNFα rescues diabetes-induced apoptosis and anti-proliferation in MSCs. Femoral calluses 16 days after fracture in normoglycaemic and diabetic mouse groups treated with or without PEG were double-stained with CD271 antibody and apoptotic marker (TUNEL) or proliferation marker (Ki67). (a) TUNEL⁺ cells per total cell number; (b) CD271⁺TUNEL⁺ cells per total CD271⁺ cells; (c) Ki67⁺ cells per total cell number; (d) CD271⁺Ki67⁺ cells per total CD271⁺ cells. n=6 each. *p<0.05 vs normoglycaemic and diabetic + pegsunercept; ^†p<0.05 vs normoglycaemic. Diab, diabetic; NG, normoglycaemic; PEG, pegsunercept