Fig. 5.

TNFα induces apoptosis and reduces proliferation in mMSCs and hBMSCs. C3H10T1/2 cells (mMSC) and hBMSCs were treated with TNFα (10 ng/ml) for 24–72 h. Apoptosis was detected using annexin V and cleaved caspase-3 antibody, and proliferation was measured by the BrdU assay. (a, d) Annexin V⁺ cells per total cell number in mMSCs and hBMSCs, respectively. (b, e) Cleaved caspase-3⁺ cells per total cell number after 72 h of TNFα treatment in mMSCs and hBMSCs, respectively. (c, f) BrdU levels after serum-stimulated proliferation, measured at absorbance = 450 nm. *p<0.05 vs 0 h treatment