Fig. 6.

TNFα inhibition reduces FOXO1 nuclear localisation in diabetic fractures in vivo. Immunofluorescence was carried out in femoral calluses 16 days after fracture in NG and diabetic mouse groups treated with or without PEG using Sca-1 and/or FOXO1 antibody. FOXO1⁺ and Sca-1⁺FOXO1⁺ cell numbers were examined. Nuclear localisation of FOXO1 was determined by the co-localisation of FOXO1 and DAPI stain. (a) Nuclear FOXO1⁺ cells per total cell number; (b) total FOXO1⁺ (cytoplasm or nucleus) per total cell number; (c) FOXO1⁺ cells per total Sca-1⁺ cells; (d) the ratio of Sca-1⁺nuclear FOXO1⁺ cells to Sca-1⁺total FOXO1⁺ cells.n=6 each. *p<0.05 vs all groups. Diab, diabetic; NG, normoglycaemic; PEG, pegsunercept