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. 2015 Feb 23;8:59–69. doi: 10.2147/JIR.S78631

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© 2015 Kubat et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The effect of PEMF treatment on heme oxygenase mRNA levels in HEK, HDF, and HMNC in culture.

Notes: Cells were treated with PEMF for 30 minutes, after which total RNA was isolated and mRNAs quantitated at several time points by qRT-PCR. Cells were harvested at the time points indicated (A) or 2 hours postinitiation of PEMF treatment (B). In (A), the x axis indicates the amount of time passed after the initiation of PEMF treatment, with the end of the 30-minute treatment period indicated by an arrow. In (B), the results of three independent experiments with PEMF are shown. All data is expressed relative to untreated control cell cultures.

Abbreviations: HDF, human dermal fibroblasts; HEK, human epidermal keratinocytes; HMNC, human mononuclear cells; mRNA, messenger RNA; PEMF, pulsed electromagnetic field; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.