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Perturbations of Sma0114 1H-15N resonances due to metal-binding. Bar graphs show composite HN and N chemical shift changes (ΔHN + 0.1(ΔN)) that occur in the presence of 1.5 mM CaCl2 (A) or 150 mM MgCl2 (B). Residues in boxes indicate amino acids that experience large chemical shift perturbations upon metal binding. Arrows represent residues that broaden beyond detection in the presence of the metals.
