Table 1. Analysis of the medians of comet, head, and tail parameters in the control, the H2O2-treated and the Fenton reagent-treated U937 cells.
Comet | |||||
---|---|---|---|---|---|
Length [μm] | Height [μm] | Area [μm2] | Intensity [r.u.] | Mean Intensity [r.u.] | |
Control | 20 ± 2 | 18 ± 2 | 301 ± 71 | 71506 ± 18571 | 51 ± 5 |
H2O2 | 44 ± 2 | 24 ± 2 | 762 ± 66 | 99182 ± 7767 | 27 ± 1 |
Fenton | 51 ± 6 | 25 ± 3 | 301 ± 71 | 123451 ± 18571 | 51 ± 5 |
Head | |||||
Diameter [μm] | %DNA | Area [μm2] | Intensity [r.u.] | Mean Intensity [r.u.] | |
Control | 18 ± 2 | 91 ± 4 | 262 ± 48 | 65320 ± 16978 | 52 ± 7 |
H2O2 | 13 ± 1 | 30 ± 2 | 178 ± 15 | 27219 ± 1385 | 31 ± 5 |
Fenton | 10 ± 1 | 21 ± 10 | 141 ± 32 | 24705 ± 7742 | 31 ± 2 |
Tail | |||||
Length [μm] | %DNA | Area [μm2] | Intensity [r.u.] | Mean Intensity [r.u.] | |
Control | 2 ± 1 | 8 ± 4 | 31 ± 21 | 5888 ± 3993 | 42 ± 9 |
H2O2 | 30 ± 3 | 69 ± 3 | 524 ± 36 | 64238 ± 9192 | 26 ± 1 |
Fenton | 39 ± 8 | 78 ± 10 | 659 ± 161 | 94581 ± 32988 | 29 ± 2 |
Data are presented as mean values and standard deviations. The mean value represents the average value from at least three measurements.