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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2014 Dec 24;265:59–68. doi: 10.1016/j.expneurol.2014.12.014

Figure 2. Immunocytochemical localization of IP3Rs in primary cultured rat ONHAs.

Figure 2

Representative images are shown for staining against IP3R1 (A), IP3Rs (B), and IP3R3 (C), using AlexaFluor® 488-labeled secondary antibody. Cells were co-stained using AlexaFluor® 647 Phalloidin, labeling actin filaments, and with the nuclear label Hoechst 33258. Note the punctate, cytosolic immunoreactivity indicative of ER localization for IP3R1, compared to the strong nuclear immunoreactivity of IP3R2. We did not detect significant IP3R3 immunoreactivity in primary cultured rat ONHAs. Scale bars are 25 µm.