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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2014 Dec 24;265:59–68. doi: 10.1016/j.expneurol.2014.12.014

Table 1.

Antibodies used for immunocytochemistry

Epitope Host Cat# Supplier Dilution Reference
anti-GFAP Rabbit Ab4674 AbCam, Cambridge, MA 1:2,000
Anti-IP3R1 Goat sc-6093 Santa Cruz Biotechnology, Santa Cruz, CA 1:50 (Payne et al., 2013a)
Anti-IP3R2 Goat sc-7278 Santa Cruz Biotechnology, Santa Cruz, CA 1:50 (Payne et al., 2013a)
Anti-IP3R3 Goat sc-7277 Santa Cruz Biotechnology, Santa Cruz, CA 1:50 (Payne et al., 2013a)
anti-RyR1 Rabbit AB9078 Chemicon; EMD Millipore, Bellerica, MA 1:15,000 (Medina-Ortiz et al., 2007;
Payne et al., 2013a)
anti-RyR2 Rabbit AB9080 Chemicon; EMD Millipore, Bellerica, MA 1:15,000 (Medina-Ortiz et al., 2007;
Payne et al., 2013a)
anti-RyR3 Rabbit AB9082 Chemicon; EMD Millipore, Bellerica, MA 1:15,000 (Medina-Ortiz et al., 2007;
Payne et al., 2013a)

We used the above subtype-specific antibodies to determine the localization and distribution of IP3Rs and RyRs in primary cultured rat ONHAs. All antibodies are well-characterized and their specificity for their respective epitopes has been shown previously.