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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Jan 14;80:126–135. doi: 10.1016/j.yjmcc.2014.12.024

Figure 4.

Figure 4

Time and dose-dependent effects of Fab on the intracellular Ca2+ activity. Confocal Ca2+ imaging of permeabilized VMs used Fluo-4 Ca2+ indicator under the line-scan mode. Baseline was obtained in the absence of Fab under the free [Ca2+] of 200 nM. A. Ca2+ images was obtained at various time points (top) after Fab exposure (100 µg/ml). (B) Ca2+ images was obtained after addition of Fab sequentially (top) at the time interval of 15 min. Plots were average of at least 4 VMs.