Figure 5.

Effect of Fab on the intracellular Ca²⁺ activity at various Ca²⁺ concentrations. A–F, At each Ca²⁺ concentration indicated ([Ca]), confocal line-scan images of the same permeabilized VM were obtained at baseline (a), after addition of 100 µg/ml Fab (b), and after 20 µM cAMP (c) exposure consecutively. Bar graphs showed the frequency of Ca²⁺ sparks or waves at each condition. P value was indicated. Traces in B to F showed intensity of fluorescent signals (F) of SCWs. See Table 2 for the Ca²⁺ decay time and velocity of SCWs (as arrows). At least 12 VMs were imaged at each condition.