Table 2.

Spontaneous Ca²⁺ wave Characteristics

	decay time (ms)			velocity (um/sec)			frequency (Hz)			ΔF/Fo
Free [Ca2+] (nM)	Baseline	Fab	Fab + cAMP	Baseline	Fab	Fab + cAMP	Baseline	Fab	Fab + cAMP	Baseline	Fab	Fab + cAMP
100	--	44±6*	47±8^	--	133±21*	138±25^	--	0.44±0.16*	0.46±0.13^	--	4.68±0.54*	4.84±0.69^
200	--	45±7*	46±9^	--	140±26*	142±20^	--	1.05±0.21*	1.07±0.21^	--	4.49±0.63*	4.45±0.57^
400	89±14	52±8*	53±9^	112±26	144±13*	146±21^	1.12±0.38	1.61±0.55*	1.76±0.62^	2.89±0.31	4.41±0.41*	4.38±0.38^
500	74±12	54±4*	52±8^	123±10	143±23*	150±25^	2.38±0.11	2.82±0.24*	2.76±0.16^	2.39±0.51	2.91±1.02	2.88±0.71
1000	61±9	60±8	60±9	157±23	156±24	157±24	2.90±0.11	2.95±0.12	2.93±0.14	2.33±0.38	2.38±0.32	2.41±0.34

Spontaneous Ca²⁺ wave (SCWs) were measured in permeabilized VMs at various free [Ca²⁺] as indicated in Figure 5. SCWs characteristics at baseline, after Fab (100µg/ml), and after cAMP (20µM) are reported. SCWs Ca²⁺ decay time, velocity, frequency, and ΔF/F0 were compared using one way ANOVA (12 cells from 4 mice were chosen for comparison in each free [Ca²⁺], * baseline vs Fab P < 0.05, ^ baseline vs Fab+cAMP P < 0.05). Data are mean±SEM.