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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2015 Jan 3;265:84–93. doi: 10.1016/j.expneurol.2014.12.018

Figure 2. MiRNA machinery proteins Dicer and AGO2 were present in rat brain mitochondria.

Figure 2

Isolated mitochondria and mitochondrial subfractions were analyzed for Dicer and AGO2 using Western blotting. Antibodies against marker proteins for the mitochondria subfractions included VDAC (outer membrane), CoxIV (inner membrane), and PDH (matrix). Brain Homog.: total brain homogenate; Total MT: total mitochondria lysate; MS: mitochondrial supernatant containing broken outer membrane and inter-membrane space compartments; OM: outer membrane; IMS: inter-membrane space; MP: mitoplast; IM: inner membrane; MTX: mitochondria matrix.