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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2015 Jan 3;265:84–93. doi: 10.1016/j.expneurol.2014.12.018

Figure 3. Enrichment of mitochondrial miRNA in AGO complexes.

Figure 3

Co-immunoprecipitation (co-IP) with anti-AGO 2A8 antibody was performed using isolated total mitochondrial lysate. Corresponding cytosols were also subjected to co-IP. The co-IP RNAs were extracted and analyzed using RT-qPCR TaqMan® assays for miR-23a, miR-107, and miR-124a, and a control small nuclear RNA U6. MiRNAs were specifically enriched in anti-AGO 2A8 antibody co-immunoprecipitates (IP2A8), while co-IP using normal mouse serum (IPNMS, data not shown) did not result in any specific signal. As expected, U6, which does not associate with AGO complexes was not detected in co-IP RNA samples. Inset: Western blot analysis of the IP products using anti-AGO 2A8 antibody (IP2A8) or normal mouse serum (IPNMS). TL: total lysate.