Co-immunoprecipitation (co-IP) with anti-AGO 2A8 antibody was performed using isolated total mitochondrial lysate. Corresponding cytosols were also subjected to co-IP. The co-IP RNAs were extracted and analyzed using RT-qPCR TaqMan® assays for miR-23a, miR-107, and miR-124a, and a control small nuclear RNA U6. MiRNAs were specifically enriched in anti-AGO 2A8 antibody co-immunoprecipitates (IP2A8), while co-IP using normal mouse serum (IPNMS, data not shown) did not result in any specific signal. As expected, U6, which does not associate with AGO complexes was not detected in co-IP RNA samples. Inset: Western blot analysis of the IP products using anti-AGO 2A8 antibody (IP2A8) or normal mouse serum (IPNMS). TL: total lysate.