Animals were injected i.p. with saline, FLX (20 mg/kg), or KET (10 mg/kg) at 10:00am and sacrificed two hours later. The prelimbic cortex (D and F) demonstrated increased cFos expression following administration of both drugs compared to saline (B), whereas the anterior cingulate cortex did not (A, C and E). Scale bar in panel E indicates 0.25mm. G-H. Animals were administered saline (n=6), FLX (20 mg/kg, n=5) or KET (10 mg/kg, n=5) at 10:00am and sacrificed two hours later. FLX and KET selectively activate the vmPFC, and the deep layers of this region, similar to DMI. ‘*’, p<0.05; ‘**’, p<0.01.