Cells in the NAc are also activated by DMI (10 mg/kg) (C) compared to animals that received saline (B). However, cFos staining was reduced when DMI was injected into animals with neuronal lesions in the vmPFC (A, E). mPFC: Bregma +3.5mm; NAc: Bregma +2.0mm. Scale bars in A and E are 0.5mm. G. Sham- and vmPFC-lesioned animals were administered saline or DMI (10 mg/kg) i.p. (10:00am) and sacrificed two hours later. In intact animals, the number of cells that were stained by cFos in the NAc was significantly greater in animals that received DMI (n=5) compared to those that received saline (n=5). However, the levels of cFos expression in the core and shell were reduced by vmPFC lesions (n=6). n=6 for lesion group that received saline injection. ‘*’, p<0.05.