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. 2015 Feb 27;47(2):e143. doi: 10.1038/emm.2014.127

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Copyright © 2015 KSBMB.

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PI-103 downregulates the level of β-TrCP1 protein via proteasome-dependent manner. (a) Cells were treated with 10 μM of PI-103 for 20 h and further incubated for 4 h in the presence of different concentrations of MG-132. (b) Cells were treated with 10 μM of PI-103 for 20 h and further incubated for 4 h in the presence of different concentrations of bafilomycin A1 or MG-132. (c) Cells were treated with PI-103 for indicated time. (a–c) Western blot analysis was performed with indicated antibodies. β-actin was used as a loading control.