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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Surg Res. 2014 Oct 8;194(1):262–272. doi: 10.1016/j.jss.2014.10.009

Figure 7.

Figure 7

AICAR minimized endothelial cell activation, and neutrophil adhesion. A, B. Mouse Kidney Glomerular Endothelial Cells were pretreated with or without AICAR for 1 h, and LPS (10ng/mL) was added to the cell culture. LPS induced upregulated expression of ICAM-1(A.), and VCAM-1 (B.). AICAR pretreatment minimized the upregulation of ICAM-1 and VCAM-1. (Nucleus=blue, ICAM-1, VCAM-1=red). C. Bone marrow derived neutrophils were added to Mouse Kidney Glomerular Endothelial Cell culture with or without AICAR pretreatment, and stimulated with LPS for 6 hours. Neutrophil adhesion was increased in vehicle treated cells (*P<0.05 vs control). AICAR pretreatment minimized the number of adhered neutrophils when compared to LPS stimulated cells (#p<0.05 vs LPS alone).