Figure 5.

Hypoxia and high glucose mediated ERK activation and Siah-1 production was required for synaptophysin degradation in neuronal cells. A. Inhibition of ERK activation with U0126 or PD98059 led to a significant suppression of Hypoxia and high glucose induced upregulation of Siah-1. Consistent with this result, inhibition of ERK activation also blocked the decline of synaptophysin under hypoxia and high glucose condition. B-D. Densitometric quantification of ERK Siah-1 and Synaptophysin in hippocampus neuronal cells exposed to high glucose under hypoxia condition. Data are mean ± SD from six independent experiments. *, P<0.05 vs. control group.