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. 2015 Jan 15;7(1):15–27.

Figure 6.

Figure 6

siRNA-mediated knockdown of Siah-1 in primary cultured hippocampus neurons. Primary culture hippocampus neurons were transfected with different concentrations of Siah-1-siRNA indicated for 48 hours. Cells without transfection or transfected with scramble siRNA (Scra-siRNA) were used as controls. A. Quantitative real-time RT-PCR was performed to quantify the extent to which endogenous Siah-1 was knocked down. Data are mean ± SD from three independent experiments. *P<0.05 compared to scramble siRNA. B. Representative immunoblots of Siah-1 showing knockdown of Siah-1 by the specific Siah-1 siRNA in a dose-dependent manner but not by the scramble siRNA. C. Densitometric quantification of Siah-1 immunoblots in hippocampus neurons transfected with scramble siRNA or Siah-1 specific siRNA. Data are mean ± SD from three independent experiments. *, P<0.05 compared with scramble siRNA.