FIG. 4.

Functional properties of hESC-derived motoneuron. (A) Confocal images of synapsin I and β-III tubulin colocalize staining (scale bar=40 μm). (B) Synaptic vesicles loaded with FM1-43 Dye of the control and 500 nM Fe³⁺ (scale bar=80 μm). (C) Cytocyolic fluorescence of the control (red circle) and 500 nM Fe³⁺ (blue circle) treatment groups loaded with FM1-43 dye and stimulated with 50 μM glutamic acid, 200 μM acetylcholine, and potassium (90 mM) solution to observe cell synaptic recycling responses to different natural agonist (n≥3, **p<0.005). Photo bleaching was tested to normalize differences (black circle). Color images available online at www.liebertpub.com/tec