FIG 2.

Analysis of the lipids and proteins in purified lipid droplets (LDs) from R. toruloides. (A) Thin-layer chromatography analysis of lipid samples from whole cells (C) and LDs (L) prepared from cultures in YPD, nitrogen-limited (NL), or phosphorus-limited (PL) media. Solid lines and dotted lines on the left of the three panels indicate lipid standards and unknown lipid species, respectively. T, triacylglycerol; F, fatty acid; D, diacylglycerol; M, monoacylglycerol; E, phosphatidylethanolamine; C, phosphatidylcholine. (B) SDS-PAGE analysis of proteins from LD, membrane, cytosol, and postnuclear supernatant (PNS) fractions. (C) SDS-PAGE analysis of LD proteins isolated from three independent samples cultured in YPD medium for 24 h. The whole lane of the YPD-24 h-3 sample was cut into 29 slices and analyzed by LC-MS/MS, and the major proteins of each band are listed. (D) SDS-PAGE analysis of LD proteins isolated from three independent samples cultured in NL medium for 24 h or PL medium for 48 h and comparison of the protein patterns with those from a YPD culture. The seven marked bands were cut for MS analysis, and the major proteins present in each band are listed on the right.