TABLE 3.

LOH quantification in the four candidate overexpression mutants validated in the secondary LOH screen

Straina	LOH quantification on Ch4
	Bfp+ Gfp− cells			Bfp− Gfp+ cells
	Mean frequency of LOH ± SEM (×10−4)	Fold changeb	P valuec	Mean frequency of LOH ± SEM (×10−4)	Fold changeb	P valuec
CEC3989 (control)	1.3 ± 0.5			0.7 ± 0.3
PTET-CDC20	42.0 ± 11.0	32	≤0.01	26.8 ± 7.9	38	≤0.01
PTET-RAD53	17.0 ± 6.0	13	≤0.01	9.1 ± 1.5	13	≤0.01
PTET-BIM1	12.5 ± 1.7	10	≤0.01	9.0 ± 2.9	13	≤0.01
PTET-RAD51	8.7 ± 1.4	7	≤0.01	5.3 ± 1.3	8	≤0.01

^aAll strains were grown on YPD + Atc.

^bRatio of the LOH frequency in a given mutant to that in control strain CEC3989.

^cMann-Whitney test.