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Verification of a/a cph1Δ/cph1Δ and a/a tec1Δ/tec1Δ deletion mutants by PCR amplification and DNA sequencing. (A) Primer sites for amplification and predicted deletion regions. (B) PCR amplification products of wild-type and mutant strains. (C and D) Deleted regions of proteins revealed by DNA sequencing. The same respective sequencing data were obtained for the two independent deletion derivatives of CPH1 and those of TEC1. The deduced deletions in the final proteins are presented.
