FIG 5.

Long hyphae are oriented vertically in a/a P37005 and a/a cph1Δ/cph1Δ biofilms, whereas short hyphae are disorganized in a/a tec1Δ/tec1Δ biofilms. Eighty x-y images (0.25-mm step intervals) representing a distance of 20 μm were extracted from the midregion of the LSCM x-y z-stack collected for each biofilm. Each panel includes top views of the extracted stack for three representative biofilms of a strain and a vertical view, with a red band representing the extracted stack. Preparations were stained with calcofluor white. (A) a/a P37005. (B) a/a cph1Δ/cph1Δ mutant. (C) a/a tec1Δ/tec1Δ mutant. The punctate patterns in panels A and B reflect a vertical orientation, and the short targeted sections in panel C reflect a random orientation. Bars = 20 μm.