TABLE 1.

Effects of minority opaque cells and temperature on thicknesses of 48-h biofilms formed by a/a control, a/a Δcph1/Δcph1, and a/a Δtec1/Δtec1 cells

Temp (°C)	Strain	Expt	Addition of opaque cells	Biofilm thickness (μm) (mean ± SD)	% increase in thickness	P value for difference with and without opaque cells	P value for difference between 28°C and 37°C
37	a/a P37005	1	−	103 ± 3			<0.0001
	a/a P37005	2	−	99 ± 4
	a/a P37005	1	+	118 ± 10	+15	0.003	<0.0001
	a/a cph1Δ/cph1Δ	1	−	104 ± 3			<0.0001
	a/a cph1Δ/cph1Δ	2	−	100 ± 2
	a/a cph1Δ/cph1Δ	1	+	121 ± 4	+16.4	0.001	<0.0001
	a/a tec1Δ/tec1Δ	1	−	60 ± 6			<0.0001
	a/a tec1Δ/tec1Δ	2	−	54 ± 6
	a/a tec1Δ/tec1Δ	1	+	61 ± 6	+2	NSa	<0.0001
28	a/a P37005	1	−	82 ± 2
	a/a P37005	1	+	90 ± 2	+10	0.001
	a/a cph1Δ/cph1Δ	1	−	95 ± 3
	a/a cph1Δ/cph1Δ	1	+	99 ± 5	+4	0.027
	a/a tec1Δ/tec1Δ	1	−	49 ± 2
	a/a tec1Δ/tec1Δ	1	+	52 ± 2	+4	0.01

^aNS, not significant.