Characterization of cell lysis frequencies of adhered germlings in Δlfd-2, Δlfd-1; Δlfd-2, and ΔPrm1; Δlfd-2 mutants under normal and altered calcium conditions. (A) Fusion (black bars), lysis (white bars), and blocked fusion (nonfused; gray bars) frequencies of adhered Δlfd-2 germlings at different calcium (Ca2+) concentrations. B) Fusion (black bars), lysis (white bars), and blocked fusion (gray bars) for single (Δlfd-1 or Δlfd-2) and double (Δlfd-1; Δlfd-2) mutants under different calcium concentrations. (C) Fusion (black bars), lysis (white bars), and blocked fusion (gray bars) of adhered germlings from single mutants (ΔPrm1 or Δlfd-2) versus the double mutant (ΔPrm1; Δlfd-2) under different calcium (Ca2+) concentrations. Bars indicate standard deviations.